Sodium Chloride → 2-Isobutyl-3-ketoglutarate diethyl ester

HRID 1793657

반응식

HRID 1793657 반응식
P([O-])([O-])([O-])=O.[K+].[K+].[K+].[Na+].[Cl-].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O.[CH2:23]([O:25][C:26](=[O:41])[CH:27]([CH2:37][CH:38]([CH3:40])[CH3:39])[C:28](=[O:36])[C:29](=O)[C:30]([O:32][CH2:33][CH3:34])=[O:31])[CH3:24]>C(OCC)(=O)C.OCC(CO)O.CS(C)=O>[CH2:23]([O:25][C:26](=[O:41])[CH:27]([CH2:37][CH:38]([CH3:39])[CH3:40])[C:28](=[O:36])[CH2:29][C:30]([O:32][CH2:33][CH3:34])=[O:31])[CH3:24]

참가 물질

8

반응물 4

HCID 5234Sodium ChlorideHCID 62657Potassium Phosphate, TribasicHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2회HCID 220229102-Isobutyl-3-ketoketoglutarate diethyl ester

생성물 1

HCID 99926592-Isobutyl-3-ketoglutarate diethyl ester

시약·촉매·용매 3

HCID 679Dimethyl Sulfoxide용매HCID 753Glycerol용매HCID 8857Ethyl Acetate용매

실험 절차

To 40 mL of 300 mM potassium phosphate buffer buffer, pH=6.5, containing NaCl (100 mM), DMSO (3% v:v), glucose (200 mM), and glycerol (10% v:v), 40 mM of 2-isobutyl-3-ketoketoglutarate diethyl ester was added along with 100 mg of lyophilized KRED 1001 and 30 mg of glucose dehydrogenase. The reaction mixture was incubated for 48 hours at 37 C. Gas chromatographic analysis showed that the yield of hydroxy diester product was greater than 80%. The product was isolated by extraction of the reaction mixture with ethyl acetate and purification using silica gel chromatography. Isolated yield was in the range of 85–95%.

후처리

  1. customIsolated
  2. customyield

출처 및 근거

ORD 기록
ord-cb2716e4893e47f5b420d1a47f407af7
출처 데이터셋
uspto-grants-2006_07
특허 번호
US07081535B2

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