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Ammonia → (2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

HRID 1826566
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HRID 1826566 반응식
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].S(O)(O)(=O)=O.[NH3:18].OP([O-])(O)=O.[K+].[NH2:25][C@H:26]([C:31]([OH:33])=[O:32])[C@H:27]([CH2:29][CH3:30])C.CC1[N+](CC2C=NC(C)=[N:45][C:46]=2[NH2:47])=CSC=1CCO.N>O>[NH2:25][C@H:26]([C:31]([OH:33])=[O:32])[CH2:27][CH2:29][CH2:30][NH:47][C:46](=[NH:45])[NH2:18].[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4]
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참가 물질

9

반응물 6

HCID 222AmmoniaHCID 1130ThiamineHCID 6306L-IsoleucineHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 516951Monobasic potassium phosphateHCID 16211166Azane;sulfuric acid

생성물 2

HCID 107526
(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal
HCID 178418600HCID 178418600, 화합물

시약·촉매·용매 1

HCID 962Water용매

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온도
32 °C

실험 절차

The novel strain 382 and its parent strain 237 were cultivated with shaking at 32° C. for 8 hours in L-broth. Then, 60 ml of the resulted seed culture were inoculated into 1 liter jar fermenter containing 0.5 L of the a fermentation media, followed by culturing with stirring at 700 rpm at 32° C. and aeration rate 0.5 liter/min. The fermentation medium contained 100 g glucose, 9 g ammonia sulfate, 1 g KH2PO4, 0.4 g MgSO4.7H2O 0.02 g FeSO4 .7H2O, 0.02 g MnSO4.7H2O, 0.2 g total nitrogen of Soybean hydrolysate, 0.3 g L-isoleucine, 0.4 mg thiamine in 1 liter of tap water (pH 7.0). During the cultivation, ammonia solution (4.7 M) was added to adjust pH to 7.0 and to supply a nitrogen source. The cultivation was carried out for 42 hours. The accumulated amount of arginine in the culture medium and the yield from glucose are shown in Table 3.

후처리

  1. additioncontaining 0.5 L of the a fermentation media
  2. stirringwith stirring at 700 rpm at 32° C.
  3. waitThe cultivation was carried out for 42 hours

출처 및 근거

ORD 기록
ord-098ac963fe8847ca95c9e9aaf13cacda
출처 데이터셋
uspto-grants-2006_05
DOI
10.6084/m9.figshare.5104873.v1
특허 번호
US07052884B2

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