[CH2:1]([O:3][C:4]([C:6]1[C:15](=[O:16])[N:14]2[C:9]([C:10]([CH3:24])=[C:11]([C:18]3(C(O)=O)[CH2:20][CH2:19]3)[C:12]([F:17])=[CH:13]2)=[C:8]([CH:25]2[CH2:27][CH2:26]2)[CH:7]=1)=[O:5])[CH3:2].C1(P(N=[N+]=[N-])(C2C=CC=CC=2)=[O:35])C=CC=CC=1.[C:45]([OH:49])([CH3:48])([CH3:47])[CH3:46].C([N:52]([CH2:55]C)CC)C>O1CCOCC1>[CH2:1]([O:3][C:4]([C:6]1[C:15](=[O:16])[N:14]2[C:9]([C:10]([CH3:24])=[C:11]([C:18]3([NH:52][C:55]([O:49][C:45]([CH3:48])([CH3:47])[CH3:46])=[O:35])[CH2:19][CH2:20]3)[C:12]([F:17])=[CH:13]2)=[C:8]([CH:25]2[CH2:26][CH2:27]2)[CH:7]=1)=[O:5])[CH3:2]A sample of the product from Step 477d is heated with diphenylphosphoryl azide, t-butanol, triethylamine and dioxane. The solvents is removed under vacuum. The residue is dissolved in methylene chloride, washed with water and dried over MgSO4 and concentrated under vacuum. The title compound is purified by chromatography on silica gel.
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