Pyrroloquinoline Quinone → L-Ascorbic Acid

HRID 1983978

반응식

HRID 1983978 반응식
P([O-])([O-])([O-])=O.[K+].[K+].[K+].C1C2C(=O)C(=O)C3N=C(C(O)=O)C=C(C(O)=O)C=3C=2NC=1C(O)=O.[Cl-].[Cl-].[Ca+2].[CH2:36]([OH:47])[C@H:37]([OH:46])[C@@H:38]([OH:45])[C@H:39]([OH:44])[C:40]([CH:42]=[O:43])=[O:41]>O>[OH:44][C:39]1[C@@H:38]([C@@H:37]([OH:46])[CH2:36][OH:47])[O:45][C:42](=[O:43])[C:40]=1[OH:41]

참가 물질

6

반응물 4

HCID 1024Pyrroloquinoline QuinoneHCID 62657Potassium Phosphate, TribasicHCID 5284359Calcium ChlorideHCID 10921067Sorbosone, L-

생성물 1

HCID 54670067L-Ascorbic Acid

시약·촉매·용매 1

HCID 962Water용매

실험 절차

A reaction mixture consisting of 1.0 mM PMS, 25 mM potassium phosphate buffer (pH 7.0), 1.0 μM pyrroloquinoline quinone (PQQ), 1.0 mM CaCl2, 50 mM L-sorbosone and enzyme solution in a final volume of 100 μl with water is prepared just before the assay. The reaction is carried out at 30° C. for 60 min unless otherwise stated. The amount of vitamin C produced is measured at a wavelength of 264 nm by a high performance liquid chromatography (HPLC) which is coupled with a UV detector (TOSOH UV8000; TOSOH Co., Kyobashi 3-2-4, Chuo-ku, Tokyo, Japan), a dualpump (TOSOH CCPE; TOSOH Co.), an integrator (Shimadzu C-R6A; Shimadzu Co., Kuwahara-cho 1, Nishinokyo, Chukyo-ku, Kyoto, Japan) and a column (YMC-Pack Polyamine-II, YMC, Inc., 3233 Burnt Mill Drive Wilimington, N.C. 28403, U.S.A.). The amount of 2-KGA produced is measured by HPLC. One unit of the enzyme activity is defined as the amount of the enzyme that produces 1 mg vitamin C or 2-KGA in 60 min in the reaction mixture.

후처리

  1. customis prepared just before the assay

출처 및 근거

ORD 기록
ord-45cd9e72bad641ddbee09d677f513445
출처 데이터셋
uspto-grants-2009_06
특허 번호
US07544494B2

연결된 노트

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