2-Mercaptoethanol → (S)-2-amino-4-(methylthio)butane-1-sulfonic acid hydrochloride

HRID 2040509노트

반응식

HRID 2040509 반응식
C1N(CCO)CC[N:3]([CH2:10][CH2:11][S:12]([OH:15])(=[O:14])=[O:13])C1.[Mg+2].[Cl-:17].[Cl-].[Cl-].[K+].[SH:21][CH2:22][CH2:23]O.P(O[CH2:38][C@H]1O[C@@H](N2C3N=CN=C(N)C=3N=C2)[C@H](O)[C@@H]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O>OCC(CO)O>[ClH:17].[NH2:3][C@@H:10]([CH2:23][CH2:22][S:21][CH3:38])[CH2:11][S:12]([OH:15])(=[O:14])=[O:13]

참가 물질

7

반응물 5

HCID 15672-MercaptoethanolHCID 4873Potassium ChlorideHCID 59575'-AtpHCID 238314-(2-Hydroxyethyl)-1-piperazineethanesulfonic acidHCID 5360315Magnesium Chloride

생성물 1

HCID 86656462(S)-2-amino-4-(methylthio)butane-1-sulfonic acid hydrochloride

시약·촉매·용매 1

HCID 753Glycerol용매

실험 절차

The assay system is as described by Hartman M et al, 2006 Enzymatic aminoacylation of tRNA with unnatural amino acids. PNAS. 103(12):4356-4361 (the content of which is hereby incorporated by reference, particularly with respect to the experimental methods described therein). Each assay contains 40 mM Hepes (pH 7.4), 17 mM MgCl2, 45 mM KCl, 3.4 mM 2-mercaptoethanol, 6 mM ATP, 6% glycerol, 350 μM E. coli tRNA (Roche), 0.09 mg/mL BSA, 910 nM Methionyl-tRNA synthetase (MetRS) and amino acids in 100-1,000 μM range. The assay is initiated by addition of the mixture of AARS and incubated at 25-30° C. for 10 minutes. Samples are transferred to ice bath to stop the reactions and then small aliquot (2 μL) samples are precipitated using 500 μL of 5% cold TCA onto Whatmann filter papers and vacuumed and then dried under a heat lamp. Radioactivity of each sample is measured as counts per minute (CPM), as compared to controls (without the analog). Results for the S enantiomer of I-A ((S)-2-amino-4-(methylthio)butane-1-sulfonic acid hydrochloride) are shown below in Table 11.

후처리

  1. additionThe assay is initiated by addition of the mixture of AARS
  2. customSamples are transferred to ice bath
  3. customsmall aliquot (2 μL) samples are precipitated
  4. filtrationfilter papers
  5. customdried under a heat lamp

출처 및 근거

ORD 기록
ord-2eaaa0a14d1c43989f9b6ee57441060a
출처 데이터셋
uspto-grants-2013_11
특허 번호
US08580859B2

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