Dimethyl Sulfoxide → D-Galactose

HRID 2172376

반응식

HRID 2172376 반응식
CS(C)=[O:3].[OH:5][CH2:6][C:7]1([O:16][CH2:15][C@@H:13]([OH:14])[C@@H:11]([OH:12])[C@@H:9]1[OH:10])[OH:8].[NH2:17][C@H:18]([C:24]([OH:26])=[O:25])[CH2:19][CH2:20][C:21](=[O:23])[OH:22]>O.C(=O)([O-])[O-]>[NH2:17][C@H:18]([C:24]([OH:26])=[O:25])[CH2:19][CH2:20][C:21](=[O:22])[OH:23].[OH:5][CH2:6][C:7]1([O:16][CH2:15][C@@H:13]([OH:14])[C@@H:11]([OH:12])[C@@H:9]1[OH:10])[OH:8].[OH:3][CH:15]1[O:16][C@H:7]([CH2:6][OH:5])[C@H:9]([OH:10])[C@H:11]([OH:12])[C@H:13]1[OH:14].[OH:22][CH:15]1[O:16][C@H:7]([CH2:6][OH:5])[C@@H:9]([OH:10])[C@H:11]([OH:12])[C@@H:13]1[OH:14]

참가 물질

9

반응물 3

HCID 679Dimethyl Sulfoxide2회HCID 33032L-Glutamic Acid3회HCID 2723872D-Fructose2회

생성물 4

HCID 6036D-GalactoseHCID 18950D-MannoseHCID 33032L-Glutamic AcidHCID 2723872D-Fructose

시약·촉매·용매 2

HCID 962Water용매HCID 19660Carbonate Ion용매2회

실험 절차

Solutions of Glu (40 mM-4.0 μM), Fru (0.4 mM), Gal (0.4 mM) and Man (0.4 mM) in deionized water were prepared. An aliquot (2.0 mL) of the solution of TPEDB (0.1 mM) in the carbonate buffer containing 4 vol % DMSO was added into a 10 mL volumetric flask, followed by the addition of 1.0 mL of a solution of Fru (0.4 mM) and 1.0 mL of a solution of Glu (40 mM). After shaking for a few seconds, a solution of TPEDB (50 μM) with 10 mM of Glu and 0.1 mM of Fru in the carbonate buffer containing 2% DMSO was obtained. The solution was allowed to stand for 30 min before its FL measurement. The solution of TPEDB and Glu containing another saccharide (Gal or Man) was prepared by similar procedures.

후처리

  1. additionwas added into a 10 mL volumetric flask
  2. customwas obtained

출처 및 근거

ORD 기록
ord-a1162ef75de54e14a34af4a42eb8f286
출처 데이터셋
uspto-grants-2015_06
특허 번호
US09051598B2

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