NADP nicotinamide-adenine-dinucleotide phosphate → NADPH

HRID 223250

반응식

HRID 223250 반응식
C(O)C(N)(CO)CO.Cl.[CH:10]1[CH:15]=[N+:14]([C@@H:16]2[O:20][C@H:19]([CH2:21][O:22][P:23]([O:26][P:27]([O:30][CH2:31][C@H:32]3[O:36][C@@H:35]([N:37]4[C:41]5[N:42]=[CH:43][N:44]=[C:45]([NH2:46])[C:40]=5[N:39]=[CH:38]4)[C@H:34]([O:47][P:48]([OH:51])([OH:50])=[O:49])[C@@H:33]3[OH:52])([OH:29])=[O:28])([OH:25])=[O:24])[C@@H:18]([OH:53])[C@H:17]2[OH:54])[CH:13]=[C:12]([C:55]([NH2:57])=[O:56])[CH:11]=1>C(O)(C)C>[CH:43]1[N:44]=[C:45]([NH2:46])[C:40]2[N:39]=[CH:38][N:37]([C@@H:35]3[O:36][C@H:32]([CH2:31][O:30][P:27]([O:26][P:23]([O:22][CH2:21][C@H:19]4[O:20][C@@H:16]([N:14]5[CH:13]=[C:12]([C:55]([NH2:57])=[O:56])[CH2:11][CH:10]=[CH:15]5)[C@H:17]([OH:54])[C@@H:18]4[OH:53])([OH:25])=[O:24])([OH:29])=[O:28])[C@@H:33]([OH:52])[C@H:34]3[O:47][P:48]([OH:51])([OH:50])=[O:49])[C:41]=2[N:42]=1

참가 물질

4

반응물 2

HCID 5886NADP nicotinamide-adenine-dinucleotide phosphateHCID 93573Tromethamine hydrochloride

생성물 1

HCID 5884NADPH

시약·촉매·용매 1

HCID 3776Isopropanol용매

반응 조건

온도
45 °C

실험 절차

The gel was used to prepare a sepharose-alcohol dehydrogenase column (0.4×4.0 cm) which was then immersed in a water bath maintained at 45° C. A 0.01 M Tris-HCl solution (pH 9.0) containing 2.5 mg/ml NADP and 0.25 v/v isopropanol was applied to the column at a rate of 0.25 ml/min.; the treated solution was collected in a cooled ethanol precipitation vessel. Several milliliters of petroleum ether were added to 20 ml of the eluted solution and the precipitate was collected by centrifugation.

후처리

  1. customwas then immersed in a water bath
  2. customthe treated solution was collected in a cooled ethanol precipitation vessel
  3. additionSeveral milliliters of petroleum ether were added to 20 ml of the eluted solution
  4. customthe precipitate was collected by centrifugation

출처 및 근거

ORD 기록
ord-509c63b7c80e462a862f4e786f5ff757
출처 데이터셋
uspto-grants-1982_10
특허 번호
US04352885

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