Kanamycin → L-Phenylalanine

HRID 280899

반응식

HRID 280899 반응식
[CH2:1]1[C@H:6](N)[C@@H:5](O[C@H]2O[C@H](CN)[C@@H](O)[C@H](O)[C@H]2O)[C@H:4](O)[C@@H:3](O[C@H]2O[C@H](CO)[C@@H](O)[C@H](N)[C@H]2O)[C@@H:2]1N.[NH2:34][C@H:35]([C:40]([OH:42])=[O:41])[CH2:36]C(C)C.N[C@H](C(O)=O)CCCNC(=N)N.OP([O-])(O)=O.[K+].OP([O-])([O-])=O.[K+].[K+].C([O-])(=O)CC(CC([O-])=O)(C([O-])=O)O.[Na+].[Na+].[Na+].O=C[C@@H]([C@H]([C@@H]([C@@H](CO)O)O)O)O>>[NH2:34][C@H:35]([C:40]([OH:42])=[O:41])[CH2:36][C:6]1[CH:5]=[CH:4][CH:3]=[CH:2][CH:1]=1

참가 물질

8

반응물 7

HCID 6032KanamycinHCID 6106L-Leucine2회HCID 6224Trisodium citrateHCID 24450Potassium Phosphate, DibasicHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 516951Monobasic potassium phosphateHCID 178418600HCID 178418600, 화합물

생성물 1

HCID 6140L-Phenylalanine

실험 절차

The cell-suspension was transferred onto a minimum medium prepared by adding 5 μg/dl kanamycin, 10 mg/dl L-leucine, 10 mg/dl L-leucine, 10 mg/dl L-arginine, 100 mg/dl D, L-m-fluorophenylalanine and 2 g/dl agar to a basal minimum medium of pH 7.2 containing 0.6 g/dl KH2PO4, 1.4 g/dl K2HPO4, 0.2 g/dl (NH4)2SO4, 0.1 g/dl sodium citrate, 0.02 g/dl MgSO4.7H2O, and 0.5 g/dl glucose. After 3 days cultivation at 37° C., two colonies appeared on the agar-medium.

후처리

  1. customprepared

출처 및 근거

ORD 기록
ord-c3807252a2b846b6b79e083ef66b1ba9
출처 데이터셋
uspto-grants-1986_05
특허 번호
US04591562

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