Glutaraldehyde → 7-Amino-2-propylheptanoic acid
반응식
Cl.[NH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH:8]([CH2:12][CH2:13][CH3:14])[C:9]([OH:11])=[O:10].C(=O)CCCC=O.N[C@H](C(O)=O)CCCCN>P([O-])([O-])([O-])=O>[NH2:2][CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH:8]([CH2:12][CH2:13][CH3:14])[C:9]([OH:11])=[O:10]참가 물질
5
실험 절차
BSA(Bovine Serum Albumin fraction V, Armour Pharm.Co.,600 mg) was dissolved in 0.2 M phosphate buffer (pH 7.0,30 ml). To the solution was added an aqueous solution (adjusted to pH 7.0 with NaHCO3, 30 ml) of 7-amino-2-propylheptanoic acid hydrochloride (prepared in Example 1, 180 mg), followed by the dropwise addition of 0.02 M aqueous glutaraldehyde solution (30 ml). The mixture was stirred for 2 hours at ambient temperature. After adding 1 M lysine solution (pH 7.5,3.0 ml), the mixture was further stirred for an hour and dialyzed for 48 hours against 0.15 M sodium chloride solution (2 liters) at 4° C., while the outer solution was renewed four times, then dialyzed against deionized water (2 liters) for 24 hours. The dialyzed solution was lyophilized to give the conjugate (550 mg).