7-Chloro-3-methyl-1-phenyl-1,2,4,5-tetrahydro-3-benzazepin-8-ol → Dopamine

HRID 327593

반응식

HRID 327593 반응식
C1C2(N(C3C=CC=CC=3)CNC2=[O:8])CCN(CCCC(C2C=CC(F)=CC=2)=O)C1.CC([C@@]1(O)C[C@@H]2N(C[C@@H]3C4C2=CC=CC=4CCC2C=CC=CC3=2)CC1)(C)C.C[N:58]1[CH2:68][CH:67](C2C=CC=CC=2)[C:66]2[C:61](=[CH:62][C:63](Cl)=[C:64]([OH:75])[CH:65]=2)CC1.CN1C[C@H](C2C=CC=CC=2)C2C=C(O)C(Cl)=CC=2CC1>>[NH2:58][CH2:68][CH2:67][C:66]1[CH:61]=[CH:62][C:63]([OH:8])=[C:64]([OH:75])[CH:65]=1

참가 물질

5

반응물 4

HCID 50187-Chloro-3-methyl-1-phenyl-1,2,4,5-tetrahydro-3-benzazepin-8-olHCID 5265SpiperoneHCID 37459(+)-ButaclamolHCID 3036864R-(+)-8-chloro-2,3,4,5-tetrahydro-3-methyl-5-phenyl-1H-3-benzazepine-7-ol

생성물 1

HCID 681Dopamine

실험 절차

The following tritiated drugs were used as radioligands for each of the receptors tested: [3H]-Spiperone 21-24 Ci/mmol for D2 receptors and [3H]-SCH23390 75-85 Ci/mmol for D1 receptors. The radioligands were incubated with various concentrations of competing drug and the appropriate membrane source for periods of time as follows: 75 minutes at room temperature for D2 receptors or 15 minutes at 37° C. for D1 receptors. Specific binding was defined using 1 μM butaclamol (D2) or 1 μM SCH23390 (D1). In addition, the D2 assays contained 30 nM ketaserin in order to block the binding of 3H-spiperone to 5HT2 receptors.

후처리

  1. concentrationThe radioligands were incubated with various concentrations
  2. waitthe appropriate membrane source for periods of time as follows
  3. custom75 minutes
  4. customat room temperature
  5. customfor D2 receptors or 15 minutes
  6. customat 37° C.

출처 및 근거

ORD 기록
ord-5ce47448ae38482fa84af9f96e6a1620
출처 데이터셋
uspto-grants-1993_12
특허 번호
US05274003

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