Acetonitrile → 4,5-Diaminofluorescein diacetate
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[CH:1]1[C:6]([OH:7])=[CH:5][C:4]2[O:8][C:9]3[CH:26]=[C:25]([OH:27])[CH:24]=[CH:23][C:10]=3[C:11]3([O:20][C:18](=[O:19])[C:17]4[C:12]3=[CH:13][C:14]([NH2:22])=[C:15]([NH2:21])[CH:16]=4)[C:3]=2[CH:2]=1.[C:28](=[O:31])([O-])[O-].[Cs+].[Cs+].[C:34](OC(=O)C)(=[O:36])[CH3:35].[C:41](#N)C>>[CH3:35][C:34]([O:7][C:6]1[CH:1]=[CH:2][C:3]2[C:11]3([O:20][C:18](=[O:19])[C:17]4[C:12]3=[CH:13][C:14]([NH2:22])=[C:15]([NH2:21])[CH:16]=4)[C:10]3[CH:23]=[CH:24][C:25]([O:27][C:28]([CH3:41])=[O:31])=[CH:26][C:9]=3[O:8][C:4]=2[CH:5]=1)=[O:36]참가 물질
5
실험 절차
DAF-2 obtained in Example 4 was dissolved in acetonitrile containing cesium carbonate, and the solution was added with 1 equivalent of acetic anhydride and stirred at room temperature for one hour. After evaporating the solvent under reduced pressure, the residue was purified by silica gel column chromatography to obtain DAF-2 DA.