Phosphoric Acid → Phytate

HRID 467407

반응식

HRID 467407 반응식
[OH-].[Na+].P(=O)(O)(O)O.[C@@H:8]1([O:39][P:40]([OH:43])([OH:42])=[O:41])[C@@H:13]([O:14][P:15]([OH:18])([OH:17])=[O:16])[C@H:12]([O:19][P:20]([OH:23])([OH:22])=[O:21])[C@@H:11]([O:24][P:25]([OH:28])([OH:27])=[O:26])[C@@H:10]([O:29][P:30]([OH:33])([OH:32])=[O:31])[C@H:9]1[O:34][P:35]([OH:38])([OH:37])=[O:36]>O>[CH:10]1([O:29][P:30]([OH:33])([OH:32])=[O:31])[CH:11]([O:24][P:25]([OH:27])([OH:28])=[O:26])[CH:12]([O:19][P:20]([OH:22])([OH:23])=[O:21])[CH:13]([O:14][P:15]([OH:18])([OH:17])=[O:16])[CH:8]([O:39][P:40]([OH:43])([OH:42])=[O:41])[CH:9]1[O:34][P:35]([OH:37])([OH:38])=[O:36]

참가 물질

5

반응물 3

HCID 1004Phosphoric AcidHCID 14798Sodium HydroxideHCID 178527091HCID 178527091, 화합물

생성물 1

HCID 890Phytate

시약·촉매·용매 1

HCID 962Water용매

실험 절차

Soybeans were pressurized, and then oil therein was extracted, separated, and removed using n-hexane as an extraction solvent to obtain low denatured defatted soybeans. To 5 kg of the obtained low denatured defatted soybeans (nitrogen solubility index (NSI) 91) was added 35 kg of water, and the pH was adjusted to 7 using diluted sodium hydroxide solution. Next, it was extracted while stirring for one hour at room temperature, and then centrifuged at 4000 G to separate bean curd refuse and insolubles, to obtain defatted soybean milk. The pH of this defatted soybean milk was adjusted to 4.5 using phosphoric acid, and then centrifuged using a continuous centrifugal separator (decanter) at 2000 G, to obtain an insoluble fraction (acid precipitated curd) and a soluble fraction (whey). Water was added so that the acid precipitated curd became 10% by mass of solid content, to obtain an acid precipitated curd slurry. After the pH of this was adjusted to 4.0 using phosphoric acid, it was heated to 40° C. To this solution (phytic acid content 1.96% by mass; solid content, TCA solubilization ratio 4.6%) was added phytase (manufactured by NOVO, Inc.) of equivalent to 8 units per solid content, allowing the enzymes to act for 30 minutes. After the reaction, the pH of this enzyme-applied product (phytic acid content 0.04% by mass; solid content, TCA solubilization ratio substantially unchanged) was adjusted to 3.5, and heat treatment was performed at 120° C. for 15 seconds, using a continuous direct heat treatment sterilizer. This was then spray dried, to obtain 1.5 kg of low phytate soybean protein powder. The dissolution ratio of this protein (pH 3.5) was 95%.

후처리

  1. extractionoil therein was extracted
  2. customseparated
  3. customremoved
  4. customto obtain low
  5. extractionNext, it was extracted
  6. customto separate bean curd refuse and insolubles
  7. customto obtain
  8. customto obtain
  9. customan insoluble fraction (acid precipitated curd)
  10. additionWater was added so that the acid
  11. customprecipitated curd
  12. customto obtain an acid
  13. customprecipitated curd slurry
  14. temperaturewas heated to 40° C
  15. additionTo this solution (phytic acid content 1.96% by mass; solid content, TCA solubilization ratio 4.6%) was added phytase (manufactured by NOVO, Inc.) of equivalent to 8 units per solid content
  16. waitto act for 30 minutes
  17. customAfter the reaction
  18. temperatureheat treatment
  19. waitwas performed at 120° C. for 15 seconds
  20. customThis was then spray dried

출처 및 근거

ORD 기록
ord-6103723f4aa54a28b7944172c9a12d17
출처 데이터셋
uspto-grants-2014_01
특허 번호
US08623443B2

연결된 노트

불러오는 중…