Diethylenetriaminepentaacetic acid → DTPA dianhydride

HRID 5299

반응식

HRID 5299 반응식
[CH2:1]([N:12]([CH2:24][C:25]([OH:27])=[O:26])[CH2:13][CH2:14][N:15]([CH2:20][C:21]([OH:23])=O)[CH2:16][C:17]([OH:19])=[O:18])[CH2:2][N:3]([CH2:8][C:9]([OH:11])=[O:10])[CH2:4][C:5]([OH:7])=O>C(Cl)(Cl)Cl>[CH2:20]1[N:15]([CH2:14][CH2:13][N:12]([CH2:24][C:25]([OH:27])=[O:26])[CH2:1][CH2:2][N:3]2[CH2:8][C:9](=[O:10])[O:11][C:5](=[O:7])[CH2:4]2)[CH2:16][C:17](=[O:19])[O:18][C:21]1=[O:23]

참가 물질

3

반응물 1

HCID 3053Diethylenetriaminepentaacetic acid

생성물 1

HCID 100825DTPA dianhydride

시약·촉매·용매 1

HCID 6212Chloroform용매

실험 절차

A 0.1 mg/ml solution of DTPA anhydride in dry chloroform was prepared and an aliquot containing the desired quantity of DTPA was added to a reaction vessel. The chloroform was removed by evaporation with nitrogen gas at room temperature. A pH 7.0 solution of antibody (approximately 0.5 mg) in 0.05M bicarbonate buffer was added to said reaction vessel to produce a 7:1 molar ratio of anhydride to protein. The solution was incubated with shaking for one minute and the coupled antibody was recovered by passage over a Sephadex G-50 column.

후처리

  1. additionwas added to a reaction vessel
  2. customThe chloroform was removed by evaporation with nitrogen gas at room temperature
  3. additionA pH 7.0 solution of antibody (approximately 0.5 mg) in 0.05M bicarbonate buffer was added
  4. customreaction vessel
  5. customto produce a 7
  6. customthe coupled antibody was recovered by passage over a Sephadex G-50 column

출처 및 근거

ORD 기록
ord-e639ae9580b5409ba9d211431f724411
출처 데이터셋
uspto-grants-1993_09
특허 번호
US05244816

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