sodium (4R)-4-[(3R,5S,7R,8R,9R,10S,12S,13R,14R,17R)-3,7,12-trihydroxy-8,10,13-trimethyl-1,2,3,4,5,6,7,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pentanoate → Palmitic Acid

HRID 611460

반응식

HRID 611460 반응식
C[C@@H:2]([C@@H:8]1[C@@:12]2(C)[C@@H:13](O)[CH2:14][C@@H:15]3[C@@:20]4(C)[CH2:21]C[C@@H](O)C[C@H:19]4[CH2:18][C@@H:17](O)[C@@:16]3(C)[C@@H:11]2[CH2:10][CH2:9]1)[CH2:3][CH2:4][C:5]([O-:7])=[O:6].[Na+]>[Cl-].[Ca+2].[Cl-]>[C:5]([OH:7])(=[O:6])[CH2:4][CH2:3][CH2:2][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH3:19].[C:5]([OH:7])(=[O:6])[CH2:4][CH2:3][CH2:2][CH2:8][CH2:9][CH2:10][CH2:11]/[CH:12]=[CH:13]\[CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH2:19][CH2:20][CH3:21].[C:5]([OH:7])(=[O:6])[CH2:4][CH2:3][CH2:2][CH2:8][CH2:9][CH2:10][CH2:11][CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH2:19][CH2:20][CH3:21]

참가 물질

5

반응물 1

HCID 44582063sodium (4R)-4-[(3R,5S,7R,8R,9R,10S,12S,13R,14R,17R)-3,7,12-trihydroxy-8,10,13-trimethyl-1,2,3,4,5,6,7,9,11,12,14,15,16,17-tetradecahydrocyclopenta[a]phenanthren-17-yl]pentanoate

생성물 3

HCID 985Palmitic Acid수율 47%HCID 5281Stearic AcidHCID 445639Oleic Acid

시약·촉매·용매 1

HCID 5284359Calcium Chloride용매

실험 절차

To 0.5 mL of a cultured cell fraction of Pyrococcus horikoshii JCM 9974 were added 2 mg of each of these substrates, 40 μl of 100 mM sodium cholate and 100 μL of 20 mM calcium chloride. The resultant reaction solution was reacted at 75° C. overnight. From the reaction solution, a fraction containing the fatty acid produced was extracted with isooctane and then subjected to gas chromatography. As a result, a decomposition product including 47-48% of oleic acid, oleic acid, palmitic acid or linolenic acid resulted from the decomposition by phospholipase A2 and 47-48% of palmitic acid, stearic acid, oleic acid or stearic acid resulted from the decomposition by phospholipase A1 was obtained from each of the substrates.

후처리

  1. customThe resultant reaction solution
  2. customwas reacted at 75° C. overnight
  3. customproduced
  4. extractionwas extracted with isooctane

출처 및 근거

ORD 기록
ord-c8c00cdd1b30490b9b3d5e825036be32
출처 데이터셋
uspto-grants-1999_12
특허 번호
US06001626

연결된 노트

불러오는 중…