Phosphoric Acid → Isoquercetin

HRID 638762

반응식

HRID 638762 반응식
C[C@@H]1O[C@@H]([O:8][CH2:9][C@H:10]2[O:15][C@@H:14]([O:16][C:17]3[C:26](=[O:27])[C:25]4[C:24]([OH:28])=[CH:23][C:22]([OH:29])=[CH:21][C:20]=4[O:19][C:18]=3[C:30]3[CH:31]=[CH:32][C:33]([OH:37])=[C:34]([OH:36])[CH:35]=3)[C@H:13]([OH:38])[C@@H:12]([OH:39])[C@@H:11]2[OH:40])[C@H](O)[C@H](O)[C@H]1O.S(=O)(=O)(O)O.C(#N)C.P(=O)(O)(O)O>O.[OH-].[Na+]>[CH:31]1[C:30]([C:18]2[O:19][C:20]3[C:25](=[C:24]([OH:28])[CH:23]=[C:22]([OH:29])[CH:21]=3)[C:26](=[O:27])[C:17]=2[O:16][C@@H:14]2[O:15][C@H:10]([CH2:9][OH:8])[C@@H:11]([OH:40])[C@H:12]([OH:39])[C@H:13]2[OH:38])=[CH:35][C:34]([OH:36])=[C:33]([OH:37])[CH:32]=1

참가 물질

7

반응물 4

HCID 1004Phosphoric AcidHCID 1118Sulfuric AcidHCID 6342AcetonitrileHCID 5280805Rutin

생성물 1

HCID 5280804Isoquercetin

시약·촉매·용매 2

HCID 962Water용매3회HCID 14798Sodium Hydroxide용매2회

반응 조건

온도
72 °C

실험 절차

Rutin (5 g) was dispersed in about 90 ml of water. One of the various edible components (food additives) listed in Table 1 was added thereto in a proportion of 0.05 wt. % or 0.5 wt. %, and dissolved or dispersed. The mixture was heated to 72° C. The pH thereof was adjusted to 4.7 by pH adjustors (sulfuric acid, sodium hydroxide). Water was added to the mixture to a total volume of 100 ml. The reaction was initiated by introducing 1 ml of an aqueous solution of naringinase (manufactured by Amano Enzyme, Inc., trade name: naringinase “Amano”) prepared by mixing 0.02 g of naringinase (specific activity: 3000 U/g) with 10 ml of water. Five hours later, 4 μl of the reaction solution was sampled and dissolved in 50 μl N sodium hydroxide. A mixed solution of 15 volume % acetonitrile and 0.085 w/v % phosphoric acid was added (2 ml in total) thereto to stop the reaction. The reaction solution thus obtained was subjected to HPLC under the conditions described below. The amount of isoquercitrin produced was calculated based on the ratio of the peak area for isoquercitrin relative to the sum of the peak areas for rutin and isoquercitrin. The yield (%) of isoquercitrin was calculated from the amount of isoquercitrin. As a control experiment, a similar reaction was conducted in a system in which none of the edible components was present, and the isoquercitrin yield (%) was obtained in the same manner.

후처리

  1. additionwas added
  2. dissolutionin a proportion of 0.05 wt. % or 0.5 wt. %, and dissolved
  3. additiondispersed
  4. custom, trade name: naringinase “Amano”) prepared
  5. customthe reaction
  6. customThe reaction solution thus obtained

출처 및 근거

ORD 기록
ord-15abe9d8c1df4b91b314017e252d6ddc
출처 데이터셋
uspto-grants-2010_04
특허 번호
US07691425B2

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