N-Hydroxysuccinimido-d-biotin → Biotin

HRID 653385

반응식

HRID 653385 반응식
O[N:2]1[C:23](=[O:24])[NH:22][C@H:21]2[C@@H:3]1[CH2:4][S:5][C@H:6]2[CH2:7][CH2:8][CH2:9][CH:10](N1C(=O)CCC1=O)[C:11](=[O:13])[OH:12]>CS(C)=O>[OH:13][C:11]([CH2:10][CH2:9][CH2:8][CH2:7][C@H:6]1[C@@H:21]2[C@@H:3]([NH:2][C:23]([NH:22]2)=[O:24])[CH2:4][S:5]1)=[O:12]

참가 물질

3

반응물 1

HCID 129676281N-Hydroxysuccinimido-d-biotin

생성물 1

HCID 171548Biotin

시약·촉매·용매 1

HCID 679Dimethyl Sulfoxide용매

실험 절차

Dissolve 1 mg of N-hydroxysuccinimidobiotin (bio-NHS) in 1 ml dimethylsulfoxide. Add 7 ul of bio-NHS solution to protein (a 3 fold molar excess). Let sit 1 hr at room temperature. Dialyze vs PBS.

후처리

  1. customDialyze vs PBS

출처 및 근거

ORD 기록
ord-460124e2cad44e45925ff2c99d38b852
출처 데이터셋
uspto-grants-1987_06
특허 번호
US04676980

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