[CH:1]1(O)[CH:6]([OH:7])[CH:5](O)[CH:4](O)[CH:3](O)[CH:2]1O.CC1[N+](CC2C=NC(C)=NC=2N)=CSC=1CCO.[ClH:31].[Cl-:32].CC1N=CC(CO)=C(CO)C=1O.Cl.[C:46]([OH:54])(=[O:53])[C:47]1C=CC=NC=1>Cl[Hg]Cl.C(O)C>[Cl:31][C:1]1[CH:2]=[C:3]([Cl:32])[CH:4]=[CH:5][C:6]=1[O:7][CH2:47][C:46]([OH:54])=[O:53]In a preferred embodiment of the process the roots are cut into 4-7 mm small explants; are sterilized by treatment with 1% cetabelon (v/v) for a period of about 5-15 minutes, then with 70% (V/v) ethanol for 30 sec., followed by treatment with 0.1% HgCl2 (w/v) for about 1-2 min. The basal medium in step (a) is modified Murashige and Skoog's (1962) medium supplemented with 200 mg/l myoinositol, 10 mg/l each of thiamine hydrochloride and pyridoxine hydrochloride and 5 mg/l of nicotinic acid (Medium I), and medium II used in step (b) is obtained by addition of 2,4-dichlorophenoxyacetic acid (0.1-2.0 mg/l) and Kn (0.1-0.5 mg/l), that are preferably added to the basal medium for optimal callus growth.
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