Sodium Chloride → (R)-3-Hydroxy-5-phenylpentanoic acid

HRID 915730

반응식

HRID 915730 반응식
P([O-])([O-])(O)=O.[Na+].[Na+].P([O-])(O)(O)=[O:9].[K+].[Cl-].[NH4+].[Cl-].[Na+].O=[CH:19][C@@H:20]([C@H:22]([C@@H:24]([C@@H:26]([CH2:28][OH:29])O)[OH:25])O)O.[CH3:30][CH2:31][CH2:32][CH2:33][CH2:34]C1C=CC=CC=1.C(C1C=CC=CC=1CCCCC)=C>>[OH:25][CH:24]([CH2:22][CH2:20][C:19]1[CH:34]=[CH:33][CH:32]=[CH:31][CH:30]=1)[CH2:26][C:28]([OH:29])=[O:9]

참가 물질

8

반응물 7

HCID 5234Sodium ChlorideHCID 10864PentylbenzeneHCID 24203Sodium Phosphate, DibasicHCID 25517Ammonium ChlorideHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanalHCID 516951Monobasic potassium phosphateHCID 221717561-Ethenyl-2-pentylbenzene

생성물 1

HCID 11106233(R)-3-Hydroxy-5-phenylpentanoic acid

실험 절차

In a 500-ml shaking flask were charged an inorganic medium containing 6.2 g/l disodium hydrogen phosphate, 3 g/l potassium dihydrogen phosphate, 1.0 g/l ammonium chloride, 0.5 g/l sodium chloride, and 3 ml/l trace components solution and 50 ml of a medium having a composition of 1.0 g/l polypeptone (Nihon Pharmaceutical Co., Ltd.), and 1.0 g/l D-glucose. ANTIFOAM PE-M was added to the resultant mixture in the same manner as in Example 2 in a concentration of 0.5 ml/l. The obtained mixture was sterilized at high temperature and high pressure and cooled to room temperature to prepare a medium. Thereafter, n-amylbenzene, i.e. an alkane, was added thereto as a substrate so as to make a concentration of 6 mM. Also, 5-(vinylphenyl)pentane, i.e. an alkane, was further added thereto so as to make a concentration of 0.8 mM. YN21M strain, one of the novel microorganisms of the present invention, was inoculated in this shaking flask and cultured with shaking at 30° C. at 125 strokes/min. After 40 hours, the cells were recovered by centrifugation and PHA was extracted in the same manner as that in Example 1 and the obtained PHA was weighed. As a result, the polymer dry weight (PDW) was found to be 0.72 g/l. Further, the structure determination of the obtained PHA by 1H-NMR in the same manner as that in Example 1 gave a monomer unit ratio of 3-hydroxy-5-phenylvaleric acid of 80.7 mol %. Also, the monomer unit ratio of 3-hydroxy-5-(4-vinylphenyl) valeric acid was found to be 15.3 mol %. From the above, it was confirmed that PHA including a 3-hydroxy-5-phenylvalerate unit and 3-hydroxy-5-(4-vinylphenyl) valerate unit could be synthesized from n-amylbenzene and vinylphenylpentane by the YN21M strain.

후처리

  1. additionwere charged an inorganic medium
  2. customto prepare a medium
  3. customYN21M strain, one of the novel microorganisms of the present invention, was inoculated in this shaking flask
  4. stirringcultured with shaking at 30° C. at 125 strokes/min
  5. customthe cells were recovered by centrifugation and PHA
  6. extractionwas extracted in the same manner as that in Example 1
  7. customAs a result, the polymer dry weight (PDW)

출처 및 근거

ORD 기록
ord-6f703fa66af0436583b0771ff7a7edf0
출처 데이터셋
uspto-grants-2007_09
특허 번호
US07267974B2

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