Ethyl Acetate → ((2R,3R,4R,5R)-3-(benzoyloxy)-4-fluoro-5-hydroxy-4-methyltetrahydrofuran-2-yl)methyl benzoate

Temperature

-30 °C

To a 5 L of dry three-neck round-bottomed flask fit with a mechanical stirrer, addition funnel and thermometer was charged the lactone ((2R,3R,4R)-3-(benzoyloxy)-4-fluoro-4-methyl-5-oxotetrahydrofuran-2-yl)methyl benzoate) (1a, 379 g, 1.018 mol). The solid was dissolved in anhydrous THF (1.75 L) and cooled to −30° C. under a nitrogen atmosphere. A solution of lithium tri-tert-butoxyaluminohydride (1.0 M in THF, 1.527 L) was added to the lactone solution while stirring over 1 h and maintaining the −30° C. temperature. After finishing the addition, the temperature was slowly increased and the reaction was followed by TLC (lactol Rf 0.4, 30% EtOAc in hexanes). The reaction was complete after 1 h 15 min (temperature reached −10° C.). The reaction was quenched by addition of Ethyl acetate (900 mL) via addition funnel. Sat. NH4Cl (40 mL) was added at 0° C. The cloudy mixture was decanted into a 10 L round-bottomed flask. The solid residue left behind was filtered and washed with ethyl acetate (2×200 mL). The filtrate was combined with the decanted solution and the combined solution was concentrated under reduced pressure. The oily residue was dissolved in ethyl acetate (2 L) and washed with 3 N HCl (600 mL). The aqueous layer was back-extracted with ethyl acetate (3×400 mL). The combined organic layer was washed with water (3×800 mL), sat. NaHCO3 (400 mL) and brine (400 mL). The organic solution was dried over MgSO4, filtered and concentrated under reduced pressure to afford a light brown oily residue. The residue was purified by plug column (2.2 kg of 40-63 micron silica gel, packed in a 6 L sintered glass funnel, 22 cm length of silica gel, diameter 15 cm) using suction and a step-gradient of 5%, 10%, 20%, and 30% ethyl acetate in hexanes —ca 5 L of each). The product containing fractions were combined and concentrated under reduced pressure to a colorless, very thick liquid (310.4 g).

1. additionTo a 5 L of dry three-neck round-bottomed flask fit with a mechanical stirrer, addition funnel
2. temperaturemaintaining the −30° C. temperature
3. additionthe addition
4. temperaturethe temperature was slowly increased
5. waitThe reaction was complete after 1 h 15 min
6. custom(temperature reached −10° C.)
7. customThe reaction was quenched by addition of Ethyl acetate (900 mL) via addition funnel
8. additionSat. NH4Cl (40 mL) was added at 0° C
9. customThe cloudy mixture was decanted into a 10 L round-bottomed flask
10. waitThe solid residue left
11. filtrationbehind was filtered
12. washwashed with ethyl acetate (2×200 mL)
13. concentrationthe combined solution was concentrated under reduced pressure
14. dissolutionThe oily residue was dissolved in ethyl acetate (2 L)
15. washwashed with 3 N HCl (600 mL)
16. extractionThe aqueous layer was back-extracted with ethyl acetate (3×400 mL)
17. washThe combined organic layer was washed with water (3×800 mL), sat. NaHCO3 (400 mL) and brine (400 mL)
18. dry with materialThe organic solution was dried over MgSO4
19. filtrationfiltered
20. concentrationconcentrated under reduced pressure
21. customto afford a light brown oily residue
22. customThe residue was purified by plug column (2.2 kg of 40-63 micron silica gel, packed in a 6 L sintered glass funnel, 22 cm length of silica gel, diameter 15 cm)
23. additionThe product containing fractions
24. concentrationconcentrated under reduced pressure to a colorless, very thick liquid (310.4 g)

ORD record

ord-bbc29100d0254b3aaa079ca0c48afa0a

Source dataset

uspto-grants-2014_05

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08716263B2