反应详情

温度

65 °C

5-(2,4-dimethoxybenzyl)-1-(tetrahydro-2H-pyran-4-yl)-7-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)-1H-pyrazolo[4,3-c]quinolin-4(5H)-one (100 mg) obtained in Preparation Example 1(5) was dissolved in 1,4-dioxane (4 mL). 3-chloro-2-methoxy-4,6-dimethylpyridine obtained in Preparation Example 25 (472 mg), [(t-Bu)2P(OH)]2PdCl2 (4.6 mg), cesium carbonate (119 mg) and water (1 mL) were added to the solution, and the mixture was reacted using a microwave reactor at 130° C. for four hours. The reaction mixture was extracted with DCM. The organic layer was washed with brine and dried over sodium sulfate. The desiccant was removed by filtration, and the filtrate was concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (ethyl acetate/n-heptane, 50% to 100%) to give a 1:1 mixture of 5-(2,4-dimethoxybenzyl)-7-(2-methoxy-4,6-dimethylpyridin-3-yl)-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[4,3-c]quinolin-4(5H)-one and 5-(2,4-dimethoxybenzyl)-1-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[4,3-c]quinolin-4(5H)-one (76 mg). The mixture (76 mg) was dissolved in TFA (1.5 mL), and the mixture was stirred at 65° C. for three hours. The reaction mixture was concentrated under reduced pressure. DCM and a saturated aqueous sodium bicarbonate solution were added to the resulting residue, followed by extraction with DCM. The organic layer was washed with brine and dried over sodium sulfate. The desiccant was removed by filtration, and the filtrate was concentrated under reduced pressure. The resulting residue was purified by silica gel column chromatography (ethyl acetate/n-heptane, 50% to 100%) to give the title compound (22 mg).

1. additionwere added to the solution
2. customthe mixture was reacted
3. customat 130° C.
4. customfor four hours
5. extractionThe reaction mixture was extracted with DCM
6. washThe organic layer was washed with brine
7. dry with materialdried over sodium sulfate
8. customThe desiccant was removed by filtration
9. concentrationthe filtrate was concentrated under reduced pressure
10. customThe resulting residue was purified by silica gel column chromatography (ethyl acetate/n-heptane, 50% to 100%)
11. customto give
12. concentrationThe reaction mixture was concentrated under reduced pressure
13. additionDCM and a saturated aqueous sodium bicarbonate solution were added to the resulting residue
14. extractionfollowed by extraction with DCM
15. washThe organic layer was washed with brine
16. dry with materialdried over sodium sulfate
17. customThe desiccant was removed by filtration
18. concentrationthe filtrate was concentrated under reduced pressure
19. customThe resulting residue was purified by silica gel column chromatography (ethyl acetate/n-heptane, 50% to 100%)