反应详情

To a solution of 1-[4-(trifluoromethyl)pyridin-2-yl]piperazine (145 mg, 0.627 mmol), (1S,3R)-3-[(tert-butoxycarbonyl)amino]-1-isopropylcyclopentanecarboxylic acid (140 mg, 0.52 mmol) in methylene chloride (10 mL) was added benzotriazol-1-yloxytris(dimethylamino)phosphonium hexafluorophosphate (253 mg, 0.572 mmol) followed by triethylamine (0.156 mL, 1.12 mmol). After being stirred overnight, the reaction mixture was diluted with EtOAc and washed with saturated NaHCO3. The aqueous layer was extracted with EtOAc three times. The combined organic layers were dried (MgSO4), concentrated and purified by flash chromatography on silica gel (20% EtOAc/hexanes to 40% EtOAc/hexanes) to give 0.15 g of desired product. MS calculated for C24H35F3N4O3: (M+H) 485; found 385.2 (M-Boc+H).

1. washwashed with saturated NaHCO3
2. extractionThe aqueous layer was extracted with EtOAc three times
3. dry with materialThe combined organic layers were dried (MgSO4)
4. concentrationconcentrated
5. custompurified by flash chromatography on silica gel (20% EtOAc/hexanes to 40% EtOAc/hexanes)