HRID1052891

反应详情

EQUATION

反应方程式

REACTANTS

反应物

结构：NC(=O)c1ccc[n+]([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](OP(=O)(O)O)[C@@H]3O)[C@@H](O)[C@H]2O)c1

HCID5886

Adenosine 5′-(trihydrogen diphosphate), 2′-(dihydrogen phosphate), P′→5′-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium

C21H29N7O17P3+

HCID62657

Tripotassium phosphate

K3O4P

结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO

HCID107526

未命名化合物

2 次

HCID58070809

1-(4-Fluorophenyl)-5-(morpholin-4-yl)pentane-1,5-dione

C15H18FNO3

PRODUCTS

生成物

HCID23636213

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

溶剂HCID887

Methanol

CH4O

溶剂HCID8857

Ethyl acetate

C4H8O2

CONDITIONS

反应条件

温度: 31 °C

PROCEDURE

实验过程

ES-KRED-119 (3.5 gm, Chiral Vision) was dissolved in 450 ml buffer (containing 250 mM potassium phosphate, 2 gm NADP+, 330 mM D-glucose, 1 gm glucose dehydrogenase, pH 7.0). A solution of 1-(4-Fluoro-phenyl)-5-morpholin-4-yl-pentane-1,5-dione in MeOH (50 gm in 50 ml) was added. The mixture was stirred at 31° C. for 24 hrs and monitored by HPLC. Ethyl acetate (250 ml) was added and the phases were separated. The organic layer was evaporated to get 49 gms of the (S)-5-(4-Fluoro-phenyl)-5-hydroxy-1morpholin-4-yl-pentan-1-one (ee: >99%).

WORKUP

后处理

customthe phases were separated
customThe organic layer was evaporated