反应详情

74 mg (0.178 mmol, 70% pure) of the compound from Example 71A and 44 mg (0.160 mmol) of the compound of Example 23A were dissolved in 1 ml anhydrous DMF, and 81 mg (0.213 mmol) of HATU and 37 μl (0.213 mmol) of N,N-diisopropylethylamine were added in succession. The reaction mixture was stirred at RT for 30 min and then stirred into about 10 ml of water. The resulting precipitate was filtered off with suction, dissolved in about 3 ml of methanol and separated into its components by preparative HPLC (Method 33). The product fractions were combined and freed from the solvent. The residue obtained was triturated with a little dichloromethane at RT. Filtration with suction and drying of the solid under high vacuum gave 36 mg (37% of theory) of the title compound.

1. filtrationThe resulting precipitate was filtered off with suction
2. dissolutiondissolved in about 3 ml of methanol
3. customseparated into its components by preparative HPLC (Method 33)
4. customThe residue obtained
5. customwas triturated with a little dichloromethane at RT
6. filtrationFiltration with suction
7. customdrying of the solid under high vacuum