2-Oxoglutaric Acid → L-Hydroxyproline

HRID 1065998Notes

Reaction equation

HRID 1065998 reaction equation
C1N(CCS(O)(=O)=O)CC[O:3]C1.C(S(O)(=O)=O)C.[NH:19]1[CH2:26][CH2:25][CH2:24][C@H:20]1[C:21]([OH:23])=[O:22].O=C(CCC(O)=O)C(O)=O.O=C1O[C@H]([C@H](CO)O)C(O)=C1O>>[OH:3][C@H:25]1[CH2:26][NH:19][C@H:20]([C:21]([OH:23])=[O:22])[CH2:24]1

Participants

6

Reactant 5

HCID 512-Oxoglutaric AcidHCID 11668Ethanesulfonic acidHCID 781654-Morpholineethanesulfonic acidHCID 145742L-ProlineHCID 54670067L-Ascorbic Acid

Product 1

HCID 5810L-Hydroxyproline

Conditions

Temperature
100 °C

Procedure

The cells, the treated cells or the enzyme preparation are added to 240 mM MES [2- (N-morphorino) ethanesulfonic acid] buffer containing 12 mM L-proline, 24 mM 2-ketoglutaric acid, 4 mM ferrous sulfate and 8 mM L-ascorbic acid to make 250 μl in total. The mixture is kept at 35° C. for 10 minutes. The reaction mixture is heated at 100° C. for 2 minutes to stop the reaction, and the amount of trans-4-hydroxy-L-proline produced in the reaction mixture is determined by HPLC.

Workup

  1. customThe cells, the treated cells or the enzyme preparation
  2. customthe reaction

Sources & evidence

ORD record
ord-e44f3796a11142c2be1f148ef0d7a628
Source dataset
uspto-grants-2001_06
Patent number
US06242231B1

Reaction notes

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