Hydrochloric Acid → 2-Amino-7-benzyl-1H-purin-6(7H)-one

Four milliliters of N,N-dimethylformamide and 0.53 ml (2.49 equivalents) of benzyl chloride were added to 0.758 g (1.85 mmols) of triacetylguanosine, and the reaction was conducted at 60° C. for 22 hours. It was identified through liquid chromatography that 69% of the starting material remained. Accordingly, 1.60 ml (10 equivalents in total) of benzyl chloride were further added thereto, and the reaction was conducted at 60° C. for 23 hours and at 70° C. for 5 hours. The temperature was returned to room temperature, and 1.57 ml (10 equivalents) of conc. hydrochloric acid were added to the solution. The reaction was conducted for 2 hours, but the cleavage of the sugar moiety was not completed. Therefore, 1.58 ml (20 equivalents in total) of conc. hydrochloric acid were further added, and the mixture was stirred overnight at room temperature. Ten milliliters of methanol were added to the reaction solution in the slurry state. The mixture was stirred for 2 hours, and then filtered. The crystals were washed twice with 5 ml of methanol, dried at 50° C. under reduced pressure for 5 hours to give 0.444 g of 7-benzylguanine crystals in a yield of 76.4%.

1. waitthe reaction was conducted at 60° C. for 23 hours and at 70° C. for 5 hours
2. customwas returned to room temperature
3. waitThe reaction was conducted for 2 hours
4. stirringThe mixture was stirred for 2 hours
5. filtrationfiltered
6. washThe crystals were washed twice with 5 ml of methanol
7. customdried at 50° C. under reduced pressure for 5 hours

ORD record

ord-7d05b7daffb94625ae55bdd05a782186

Source dataset

uspto-grants-2001_06

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US06252075B1