反应详情

To a solution of 0.6 g 1-[5-(5-Chloro-thiophen-2-yl)-isoxazol-3-ylmethyl]-2-(1-isopropyl-piperidin-4-ylcarbamoyl)-1H-indole-5-carboxylic acid in 10 ml DMF sequentially 0.4 ml EtOH, 110 mg DMAP and 256 mg DCC were added and the reaction mixture was stirred for 16 h at room temperature. The precipitate was then filtered off and the filtrate was concentrated and purified by chromatography on silica gel eluting with DCM/MeOH/AcOH/H2O 95:3:0.5:0.5. The fractions containing the product were collected and the solvent evaporated under reduced pressure.

1. filtrationThe precipitate was then filtered off
2. concentrationthe filtrate was concentrated
3. custompurified by chromatography on silica gel eluting with DCM/MeOH/AcOH/H2O 95:3:0.5:0.5
4. additionThe fractions containing the product
5. customwere collected
6. customthe solvent evaporated under reduced pressure