Water → HCID 14814219, compound

HRID 1089289Notes

Reaction equation

HRID 1089289 reaction equation
[NH2:1][C:2]1[N:10]=[C:9]2[C:5]([N:6]=[CH:7][N:8]2[O:11][CH:12]([CH2:24][CH2:25][O:26]CC2C=CC(OC)=CC=2)[CH2:13][O:14]CC2C=CC(OC)=CC=2)=[C:4](Cl)[N:3]=1.ClC1C(=O)C(C#N)=C(C#N)C(=O)C=1Cl.C([O-])=O.[NH4+].O>ClCCl.CO.[Pd]>[NH2:1][C:2]1[N:10]=[C:9]2[C:5]([N:6]=[CH:7][N:8]2[O:11][CH:12]([CH2:24][CH2:25][OH:26])[CH2:13][OH:14])=[CH:4][N:3]=1

Participants

9

Reactant 4

HCID 962WaterHCID 67752,3-Dichloro-5,6-dicyano-1,4-benzoquinoneHCID 2723923Ammonium formateHCID 14814218HCID 14814218, compound

Product 1

HCID 14814219HCID 14814219, compoundYield 36%

Reagents, catalysts & solvents 4

HCID 23938PalladiumCatalystHCID 887MethanolSolvent2×HCID 6344DichloromethaneSolvent2×HCID 23938PalladiumSolvent

Procedure

To a solution of 2-amino-9-[1,4-bis(4-methoxybenzyloxy)but-2-oxy]-6-chloropurine (0.94 g, 1.8 mmol) in dichloromethane (7.2 ml) and methanol (0.8 ml) was added 2,3-dichloro-5,6-dicyanobenzoquinone (0.91 g, 4.0 mmol) and the solution was stirred at room temperature for 80 minutes. The solution was diluted with dichloromethane (8 ml) and extracted with water (3×8 ml). The aqueous layers were combined, filtered and the solvent removed. The residue was purified by reverse-phase column chromatography on Spherisorb C18 300 silica eluting with 10% methanol in water followed by column chromatography on silica gel eluting with chloroform-methanol (12:1). The product was suspended in a solution of ammonium formate (208 mg, 3.3 mmol) in methanol (8 ml), 10% palladium-on-charcoal was added (25 mg) and the mixture was heated under reflux. After 30 minutes further 10% palladium-on-charcoal (10 mg) was added and the mixture was heated under reflux for a further 1 hour. The mixture was allowed to cool and water (2 ml) was added. The solution was filtered and the solvent was removed. The residue was purified by reverse-phase column chromatography on Spherisorb C18 300 silica eluting with water and 10% methanol to afford 2-amino-9-(1,4-dihydroxybut-2-oxy)purine (155 mg, 36%), m.p. 178°-179° C.; UV: λmax (H2O) 222 (ε24,100) and 304 (ε7,020)nm; IR: υmax (KBr) 3330, 3210, 3070, 1655, 1640, 1580, 1510, 1480 and 1435 cm-1 ; 1H NMR: δH [(CD3)2SO] 1.84 (2H, q, J=6.4 Hz, 3'-H), 3.58 (4H, m, 1'-H and 4'-H), 4.42 (1H, m, 2'-H), 4.63 (1H, t, J=5.2 Hz, D2O exchangeable, OH), 4.98 (1H, t, J=5.9 Hz, D2O exchangeable, OH), 6.70 (2H, s, D2O exchangeable, 2-NH2), 8.23 (1H, s, 8-H) and 8.59 (1H, s, 6-H). Found: C, 45.13; H, 5.47; N, 29.30%. C9H13N5O3 requires C, 45.18; H, 5.48; N, 29.27%.

Workup

  1. extractionextracted with water (3×8 ml)
  2. filtrationfiltered
  3. customthe solvent removed
  4. customThe residue was purified by reverse-phase column chromatography on Spherisorb C18 300 silica eluting with 10% methanol in water
  5. additionwas added (25 mg)
  6. temperaturethe mixture was heated
  7. temperatureunder reflux
  8. temperaturethe mixture was heated
  9. temperatureunder reflux for a further 1 hour
  10. temperatureto cool
  11. filtrationThe solution was filtered
  12. customthe solvent was removed
  13. customThe residue was purified by reverse-phase column chromatography on Spherisorb C18 300 silica eluting with water and 10% methanol

Sources & evidence

ORD record
ord-b2e93d470d5e46b98136356845c4b71e
Source dataset
uspto-grants-1990_10
Patent number
US04965270

Reaction notes

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