Trisodium Phosphate → 2816-24-2

HRID 1155749Notes

Reaction equation

HRID 1155749 reaction equation
P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+].[CH:9]1[CH:14]=[C:13]([N+:15]([O-:17])=[O:16])[C:12]([O:18][C@@H:19]2[O:24][C@H:23]([CH2:25][OH:26])[C@H:22]([OH:27])[C@H:21]([OH:28])[C@H:20]2[OH:29])=[CH:11][CH:10]=1.C(=O)(O)[O-].[Na+]>C1(C)C=CC=CC=1>[CH:9]1[CH:14]=[C:13]([N+:15]([O-:17])=[O:16])[C:12]([O:18][CH:19]2[O:24][CH:23]([CH2:25][OH:26])[CH:22]([OH:27])[CH:21]([OH:28])[CH:20]2[OH:29])=[CH:11][CH:10]=1

Participants

5

Reactant 3

HCID 24243Trisodium PhosphateHCID 96647NitrophenylgalactosidesHCID 516892Sodium Bicarbonate

Product 1

HCID 2199732816-24-2

Reagents, catalysts & solvents 1

HCID 1140TolueneSolvent

Procedure

The assay used is based on that of Lederberg (J. Bacteriol. 1950, 60, 381), using an assay mixture comprising 2.8 ml of 50 mM sodium phosphate, pH 7.0, and 0.1 ml of 68 mM ONPG. 0.1 ml aliquots of both purified enzyme and toluene-treated cell suspension, and 10 mg. amounts in 2.9 ml of buffer of freeze dried immobilised whole cell preparation are used for the assays. Assays are carried out at incubation temperature of 65° C. and after the appropriate time interval the reactions are halted by addition of 3 ml aliquots of 0.5 M sodium bicarbonate to each reaction mixture and immediate cooling in an ice bath. The absorbance of the resultant solutions is measured at 410 nm using a Perkin-Elmer 124 double beam spectrophotometer. In the case of whole cell suspensions the assay tubes are centrifuged and in the case of the immobilised cells the mixtures are filtered, to remove cells and polyacrylamide gel respectively prior to measurement of the absorbance.

Workup

  1. customamounts in 2.9 ml of buffer of freeze dried immobilised
  2. customwhole cell preparation
  3. customare carried out at incubation temperature of 65° C.
  4. temperatureimmediate cooling in an ice bath
  5. filtrationare filtered
  6. customto remove cells and polyacrylamide gel respectively

Sources & evidence

ORD record
ord-0b0329b26d4b49dba0876bd211d399c8
Source dataset
uspto-grants-1982_06
Patent number
US04332895

Reaction notes

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