反应详情

温度

0 °C

To a mixture of [3-(3-Chloro-4-hydroxy-phenylcarbamoyl)-pyrrolidin-1-yl]-acetic acid (10 mg, 0.034 mmol) and HOBt (6.8 mg, 0.050 mmol) in DMF (0.5 ml) at 0° C. was added EDCl (9.6 mg, 0.050 mmol). The formed reaction mixture was stirred at 0° C. for 0.5 hour and then 2-(4-Pyrimidin-2-yl-phenyl)-2,5-diaza-bicyclo[2.2.1]heptane hydrochloride salt (12.6 mg, 0.05 mmol) was added. After stirred at RT for overnight, the reaction mixture was purified using a Prep-HPLC to give titled compound (3.4 mg, 19% yield). Mass Spec ES (533.1, M+H)

1. customThe formed reaction mixture
2. stirringAfter stirred at RT for overnight
3. customthe reaction mixture was purified