2-Oxoglutaric Acid → L-Hydroxyproline

HRID 1167052Notes

Reaction equation

HRID 1167052 reaction equation
C1N(CCS(O)(=O)=O)CC[O:3]C1.[NH:13]1[CH2:20][CH2:19][CH2:18][C@H:14]1[C:15]([OH:17])=[O:16].O=C(CCC(O)=O)C(O)=O.O=C1O[C@H]([C@H](CO)O)C(O)=C1O>>[OH:3][C@H:19]1[CH2:20][NH:13][C@H:14]([C:15]([OH:17])=[O:16])[CH2:18]1

Participants

5

Reactant 4

HCID 512-Oxoglutaric AcidHCID 781654-Morpholineethanesulfonic acidHCID 145742L-ProlineHCID 54670067L-Ascorbic Acid

Product 1

HCID 5810L-Hydroxyproline

Conditions

Temperature
100 °C

Procedure

The enzyme preparation to be determined is added to 80 mM MES buffer (pH 6.5) containing 4 mM L-proline, 8 mM 2-ketoglutaric acid, 2 mM ferrous sulfate and 4 mM L-ascorbic acid to make 250 μl in total, and the mixture was allowed to stand at 30° C. for 20 minutes. The reaction mixture is heated at 100° C. for 2 minutes so as to stop the reaction, and the amount of the trans-4-hydroxy-L-proline produced in the reaction mixture is determined by high performance liquid chromatography (hereinafter referred to as HPLC).

Workup

  1. customThe enzyme preparation
  2. customthe reaction

Sources & evidence

ORD record
ord-81ed51da2d31439ebd46e85ca9265aa2
Source dataset
uspto-grants-2003_09
Patent number
US06617140B2

Reaction notes

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