反应详情

LiOH.H2O (110 mg, 2.63 mmol) was added to a solution of [(thiophene-2-carbonyl)-amino]-acetic acid ethyl ester (140 mg, 0.66 mmol) in a mixture of MeOH (5 mL), THF (2.5 mL) and H2O (2.5 mL). The resulting mixture was stirred for 3 hrs at room temperature. The MeOH and THF were evaporated and the resulting residue was diluted with water (2 mL), acidified with citric acid and extracted with EtOAc. The organic layer was washed with brine solution, dried over Na2SO4 and concentrated under reduced pressure to afford [(thiophene-2-carbonyl)-amino]-acetic acid in 70% yield. LC-MS purity: 92%.

1. customThe MeOH and THF were evaporated
2. additionthe resulting residue was diluted with water (2 mL)
3. extractionextracted with EtOAc
4. washThe organic layer was washed with brine solution
5. dry with materialdried over Na2SO4
6. concentrationconcentrated under reduced pressure