反应详情

To a solution of 121 mg of 4-((S)-3-tert-Butoxycarbonyl-2-{[4-((R)-1-carboxy-ethoxy)-7-methyl-quinoline-2-carbonyl]-amino}-propionyl)-piperazine-1-carboxylic acid ethyl ester in 1 ml of DCM, 43 mg of EDC, 43 mg of pentafluorophenol and 27 mg of NEM was added and the reaction mixture was stirred for 2 h. Then, 67 mg of (S)-Pyrrolidine-2-carboxylic acid cyclobutylamide trifluoroacetate and 20 mg of NEM in 2 ml of DCM was added. After 1 h the reaction mixture was diluted with water. After filtration through a chem Elut® cartridge by eluting with ethyl acetate the solvents were removed under reduced pressure. The isolated crude product was pure enough for the next reaction step.

1. filtrationAfter filtration through a chem Elut® cartridge
2. washby eluting with ethyl acetate the solvents
3. customwere removed under reduced pressure
4. customThe isolated crude product
5. customwas pure enough for the next reaction step