反应详情

To a solution of 220 mg of (S)-4-Benzyloxy-2-tert-butoxycarbonylamino-butyric acid, 327 mg of NEM and 233 mg of TOTU in 1.6 ml of DMF, 112 mg of Piperazine-1-carboxylic acid ethyl ester was added at RT and stirred for 16 h. The reaction mixture was diluted saturated aqueous sodium hydrogen carbonate solution. After filtration through a chem Elut® cartridge by eluting with ethyl acetate the solvents were removed under reduced pressure. The isolated crude product was pure enough for the next reaction step.

1. filtrationAfter filtration through a chem Elut® cartridge
2. washby eluting with ethyl acetate the solvents
3. customwere removed under reduced pressure
4. customThe isolated crude product
5. customwas pure enough for the next reaction step