Hydrochloric Acid → 89319-60-8

HRID 1270036Notes

Reaction equation

HRID 1270036 reaction equation
[Na+].[C:2]([O:21][CH2:22][C@H:23]([CH2:44][O:45][P:46]([O:49][CH2:50][C@@H:51]([C:53]([O-:55])=[O:54])[NH2:52])([OH:48])=[O:47])[O:24]C(=O)CCCCCCC/C=C\CCCCCCCC)(=[O:20])[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9]/[CH:10]=[CH:11]\[CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH3:19].C(O)C(N)(CO)CO.Cl.[Cl-].[K+].Cl>O>[C:2]([O:21][CH2:22][C@H:23]([CH2:44][O:45][P:46]([O:49][CH2:50][C@@H:51]([C:53]([OH:55])=[O:54])[NH2:52])([OH:48])=[O:47])[OH:24])(=[O:20])[CH2:3][CH2:4][CH2:5][CH2:6][CH2:7][CH2:8][CH2:9]/[CH:10]=[CH:11]\[CH2:12][CH2:13][CH2:14][CH2:15][CH2:16][CH2:17][CH2:18][CH3:19]

Participants

6

Reactant 4

HCID 313Hydrochloric AcidHCID 4873Potassium ChlorideHCID 93573Tromethamine hydrochlorideHCID 88111507HCID 88111507, compound

Product 1

HCID 954709989319-60-8

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

After 1,2-dioleoyl-sn-glycero-3-phospho-L-serine sodium salt (SIGMA, P1060) (85 mg) was dissolved in distilled water (25 ml), 200 mM Tris-HCl buffer (pH 8.0) (1.5 ml) and 100 mM potassium chloride (1.5 ml) were added to the solution, and phospholipase A2 (0.3 ml) (10 mg/ml, 5 mM sodium acetate buffer (pH 5.0)) was further added thereto. The mixture was reacted at room temperature for 20 hours, and the pH was rendered 2 to 3 with 5M hydrochloric acid to terminate the reaction. After the reaction solution was concentrated to dryness, the residue was dissolved in a chloroform-methanol solvent mixture and the solution was adsorbed on silica gel column chromatography. Elution was performed with a chloroform-methanol solvent mixture to give 1-oleoyl-sn-glycero-3-phospho-L-serine.

Workup

  1. additionwas further added
  2. customThe mixture was reacted at room temperature for 20 hours
  3. customto terminate
  4. customthe reaction
  5. concentrationAfter the reaction solution was concentrated to dryness
  6. dissolutionthe residue was dissolved in a chloroform-methanol solvent mixture
  7. washElution

Sources & evidence

ORD record
ord-704ca18be92f42908d5ea0f2de32e3a5
Source dataset
uspto-grants-2007_04
Patent number
US07202040B2

Reaction notes

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