反应详情

To a mixture of 2-oxo-2,3-dihydrothiazol-4-ylacetic acid (0.4 g.) and N-hydroxysuccinimide (0.3 g.) in tetrahydrofuran (15 ml.) was added N,N'-dicyclohexylcarbodiimide (0.52 g.) with stirring at room temperature, and the mixture was further stirred for 2 hours at the same temperature. Thus obtained solution was added to a solution of 3-carbamoyloxymethyl-7-amino-3-cephem-4-carboxylic acid (0.816 g.) and trimethylsilylacetamide (0.27 g.) in ethyl acetate (30 ml.) at room temperature, and the mixture was stirred for 16 hours at the same temperature. After the reaction, the reaction mixture was cooled to at -20° C., and then water (20 ml.) was added thereto. After stirring the mixture sufficiently, the mixture was filtered, and then the ethyl acetate layer was separated therefrom. The ethyl acetate layer was washed with a saturated aqueous solution of sodium chloride, dried over magnesium sulfate, treated with activated carbon, and then the solvent was distilled off under reduced pressure. The residue was pulverized in diethyl ether, collected by filtration and then dried to give 3-carbamoyloxymethyl-7-(2-oxo-2,3-dihydrothiazol-4-yl)acetamido-3-cephem-4-carboxylic acid (60 mg.).

1. stirringthe mixture was further stirred for 2 hours at the same temperature
2. stirringthe mixture was stirred for 16 hours at the same temperature
3. customAfter the reaction
4. temperaturethe reaction mixture was cooled to at -20° C.
5. stirringAfter stirring the mixture sufficiently
6. filtrationthe mixture was filtered
7. customthe ethyl acetate layer was separated
8. washThe ethyl acetate layer was washed with a saturated aqueous solution of sodium chloride
9. dry with materialdried over magnesium sulfate
10. additiontreated with activated carbon
11. distillationthe solvent was distilled off under reduced pressure
12. filtrationcollected by filtration
13. customdried