反应详情

To a solution of 1,3-dipropyl-5,6-diaminouracil (2.2 mmol) in methanol was added an equimolar amount of 6-hydroxy-pyridazine-3-carboxylic acid, followed by a slight excess of DCl ((N-(3-(dimethylamino)propyl)-N′ethylcarbodiimide hydrochloride). The mixture was stirred at room temperature for 4–5 hours until complete by TLC. Water was then added and the precipitate removed by filtration. The solid was dissolved in 10% aqueous NaOH (20 mL), then heated at 70° C. for 30 minutes. After cooling to room temperature, the reaction mixture was acidified to pH=5 with 10% hydrochloric acid and the precipitated product collected by filtration.

1. customthe precipitate removed by filtration
2. dissolutionThe solid was dissolved in 10% aqueous NaOH (20 mL)
3. temperatureheated at 70° C. for 30 minutes
4. temperatureAfter cooling to room temperature
5. filtrationthe precipitated product collected by filtration