Chlorotrimethylsilane → Dinoprost

Temperature

-10 °C

Refer to Chart F, wherein E is --CH=CH--, R2, R4, and R6 are methyl, R7 is (CH3)3Si--, V is --CH=CH--(CH2)3 --, W is 1-pentyl, and ~ is alpha or beta. A solution of PGE2, methyl ester, 15-methyl ether (Example 1, 1.0 g.) in 20 ml. of dry tetrahydrofuran (THF) is stirred with 3 ml. of hexamethyldisilazane and 0.6 ml. of trimethyl chlorosilane for 20 hrs. at about 25° C., with protection from moisture. The mixture is concentrated under reduced pressure, then taken up in 50 ml. of dry benzene and again concentrated. The residue is dissolved in 150 ml. of cold methanol and treated, with stirring, with a cold (-10° C) solution of sodium borohydride (2.8 g.) in 150 ml. of methanol, maintaining the temperature below about 10° C. After 10 minutes of additional stirring, there is added 5 ml. of acetone and sufficient acetic acid to neutralize the mixture. The mixture is concentrated under reduced pressure to about 75 ml. Water (about 75 ml.) is added to hydrolyze the trimethylsilyl group thereafter stirring at about 25° C. for 3 hrs. When the trimethylsilyl group is removed, as shown by TLC, the mixture is concentrated under reduced pressure to remove most of the methanol. The remaining solution is extracted with ethyl acetate, and the combined ethyl acetate extracts are washed with brine, dried over anhydrous magnesium sulfate, and concentrated under reduced pressure. The residue is taken up in a minimum amount of dichloromethane and subjected to silica gel chromatography, eluting with 50-100% ethylacetate-Skellysolve B (isomeric hexanes). Those fractions shown to contain the 9α-hydroxy title compound free of starting compound and by-products by TLC are combined to yield the PGF2α -type Formula-VII title compound. Mass spectral peaks at 382, 364, 350, 346, and 332; infrared spectral absorptions at 3410, 1735, 1660, 1435, 1365, 1315, 1240, 1215, 1195, 1170, 1090, and 970 cm.-1 ; NMR peaks at 5.42 (multiplet), 3.9 (multiplet), 3.64, 3.49, 3.21, and 0.9 (triplet) 6.

1. customat about 25° C.
2. concentrationThe mixture is concentrated under reduced pressure
3. concentrationof dry benzene and again concentrated
4. dissolutionThe residue is dissolved in 150 ml
5. additionof cold methanol and treated
6. temperatureof methanol, maintaining the temperature below about 10° C
7. additionAfter 10 minutes of additional stirring, there is added 5 ml
8. concentrationThe mixture is concentrated under reduced pressure to about 75 ml
9. additionWater (about 75 ml.) is added
10. stirringthereafter stirring at about 25° C. for 3 hrs
11. customWhen the trimethylsilyl group is removed
12. concentrationthe mixture is concentrated under reduced pressure
13. customto remove most of the methanol
14. extractionThe remaining solution is extracted with ethyl acetate
15. washthe combined ethyl acetate extracts are washed with brine
16. dry with materialdried over anhydrous magnesium sulfate
17. concentrationconcentrated under reduced pressure
18. washeluting with 50-100% ethylacetate-Skellysolve B (isomeric hexanes)

ORD record

ord-84944d7319e44389b5fe3212831e5720

Source dataset

uspto-grants-1976_08

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US03975404