Water → 2-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

HRID 1354532Notes

Reaction equation

HRID 1354532 reaction equation
O.[CH:2]1[C:7](N=C=S)=[CH:6][C:5]2[C:11]([O:13][C:14]3([C:24]4[CH:25]=[CH:26][C:27]([OH:29])=[CH:28][C:23]=4[O:22][C:16]4[CH:17]=[C:18]([OH:21])[CH:19]=[CH:20][C:15]3=4)[C:4]=2[CH:3]=1)=[O:12]>O1CCCC1>[CH:2]1[CH:7]=[CH:6][C:5]([C:11]([OH:13])=[O:12])=[C:4]([C:14]2[C:15]3[CH:20]=[CH:19][C:18]([OH:21])=[CH:17][C:16]=3[O:22][C:23]3[C:24]=2[CH:25]=[CH:26][C:27]([CH:28]=3)=[O:29])[CH:3]=1

Participants

4

Reactant 2

HCID 962WaterHCID 18730Fluorescein 5-isothiocyanate2×

Product 1

HCID 33832-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)benzoic acid

Reagents, catalysts & solvents 1

HCID 8028TetrahydrofuranSolvent

Procedure

A PNA or PNA derivative having a single free NH2 such as H2N-GCAT-COOH is dissolved in tetrahydrofuran:water to provide a solution that is 0.1 M in PNA and to this is added fluorescein isothiocyanate to give a solution which is 0.1-1.0 M in fluorescein isothiocyanate. The solution is. stirred for 0.1-2 hours, then evaporated to a solid and purified by preparative HPLC.

Workup

  1. customto give a solution which
  2. customevaporated to a solid
  3. custompurified by preparative HPLC

Sources & evidence

ORD record
ord-638b791eb4d048a295c36645e3b114f8
Source dataset
uspto-grants-2007_05
Patent number
US07223833B1

Reaction notes

Loading…