Hydrogen Peroxide → 2-amino-9-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]purine-6,8-dione

HRID 1367450Notes

Reaction equation

HRID 1367450 reaction equation
OO.[C@@H:3]1([N:12]2[CH:19]=[N:18][C:16]([NH2:17])=[N:15][C:13]2=[O:14])[O:11][C@H:8]([CH2:9][OH:10])[C@@H:6]([OH:7])[C@H:4]1O.[C:20]([O-:25])(=O)CCC.[Na+].C(O)[C:28]([NH2:33])(CO)CO.Cl>>[O:25]=[C:20]1[N:33]=[C:28]2[C:19](=[N:18][C:16]([NH2:17])=[N:15][C:13]2=[O:14])[N:12]1[C@@H:3]1[O:11][C@H:8]([CH2:9][OH:10])[C@@H:6]([OH:7])[CH2:4]1

Participants

5

Reactant 4

HCID 784Hydrogen PeroxideHCID 9444AzacytidineHCID 93573Tromethamine hydrochlorideHCID 5222465Sodium Butyrate

Product 1

HCID 152768222-amino-9-[(2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]purine-6,8-dione

Procedure

The H2O2-UV irradiated slides or slides produced with cells grown in presence of either Cd+Cr+H2O2 or 5-azacytidine, or sodium butyrate are incubated overnight at 4° C. in the presence of 30 μl of an anti-8-oxo-dG mouse monoclonal antibody (Gentaur) diluted 1/100 in 10 mM Tris-HCl, pH 7.5, 10% Foetal Bovine Serum. The slides are washed once in PBS and then incubated for 40 minutes in the presence of a goat anti-mouse IgG1 antibody (southern Biotechnology Associates, Inc) diluted at a concentration of 0.1 μg/1×106 cells in PBS.

Workup

  1. customproduced with cells
  2. washThe slides are washed once in PBS
  3. additiondiluted at a concentration of 0.1 μg/1×106 cells in PBS

Sources & evidence

ORD record
ord-95ffac458b3f42aba270da6291194c05
Source dataset
uspto-grants-2011_02
Patent number
US07893250B2

Reaction notes

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