Triethylamine → HCID 14558097, compound

Triethylamine (13.4 ml, 100 mmol) was added dropwise to a stirred ice-cooled mixture of 1,2,4-triazole (6.22 g, 90 mmol) and phosphorous oxychloride (1.89 ml, 20 mmol) in 50 ml of anhydrous acetonitrile. To the resulting suspension the solution of 2',3',5'-tri-O-Benzoyl-6-methyl uridine (5.7 g, 10 mmol) in 30 ml of acetonitrile was added dropwise and the reaction mixture was stirred for 4 hours at room temperature. Then it was concentrated in vacuo to minimal volume (not to dryness). The residue was dissolved in chloroform and washed with water, saturated aq sodium bicarbonate and brine. The organic layer was dried over sodium sulfate and the solvent was removed in vacuo. The residue was dissolved in 100 ml of 1,4-dioxane and treated with 50 mL of 29% aq NH4OH overnight. The solvents were removed in vacuo. The residue was dissolved in the in the mixture of pyridine (60 mL) and methanol (10 mL), cooled to -15° C. and ice-cooled 2M aq solution of sodium hydroxide was added under stirring. The reaction mixture was stirred at -10° to -15° C. for additional 30 minutes and then neutralized to pH 7 with Dowex 50 (Py+). The resin was filtered off and washed with 200 mL of the mixture H2O --Py (4:1). The combined mother liquor and washings were evaporated to dryness. The residue was crystallized from aq methanol to give 1.6 g (62%) of 6-methyl cytidine.

1. temperaturecooled
2. concentrationThen it was concentrated in vacuo to minimal volume (not to dryness)
3. dissolutionThe residue was dissolved in chloroform
4. washwashed with water, saturated aq sodium bicarbonate and brine
5. dry with materialThe organic layer was dried over sodium sulfate
6. customthe solvent was removed in vacuo
7. dissolutionThe residue was dissolved in 100 ml of 1,4-dioxane
8. additiontreated with 50 mL of 29% aq NH4OH overnight
9. customThe solvents were removed in vacuo
10. dissolutionThe residue was dissolved in the
11. additionin the mixture of pyridine (60 mL) and methanol (10 mL)
12. temperaturecooled to -15° C.
13. temperatureice-cooled 2M aq solution of sodium hydroxide
14. additionwas added
15. stirringunder stirring
16. stirringThe reaction mixture was stirred at -10° to -15° C. for additional 30 minutes
17. filtrationThe resin was filtered off
18. washwashed with 200 mL of the mixture H2O
19. customThe combined mother liquor and washings were evaporated to dryness
20. customThe residue was crystallized from aq methanol

ORD record

ord-c1a4a83ca7ef423788b8583810e623b3

Source dataset

uspto-grants-1997_09

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US05672501