反应详情

温度

0 °C

Z-Arg-OH.HCl (6 g, 8.7 mmoles) and benzyl mercaptan (4.2 ml, 17.4 mmoles) are dissolved in dimethylformamide (DMF) and cooled to 0° C. DCC (3.6 g, 8.7 mmoles), dissolved in DMF, is then added to the above reaction mixture, stirred at 0° C. for 30 minutes, then stirred overnight at room temperature. The precipitate formed is removed by filtration and the filtrate is concentrated under vacuum to yield an oil. This oily residue is dissolved in ethyl acetate and washed, successively, with 0.1 N HCl, 5% NaHCO3, and saturated aqueous NaCl. The organic layer is dried over anhydrous Na2SO4 and the solvent removed by evaporation. The crude product is purified by silica gel column, using a CHCl3 /methanol system, yielding 4.5 g of the product.

1. additionis then added to the above reaction mixture
2. stirringstirred overnight at room temperature
3. customThe precipitate formed
4. customis removed by filtration
5. concentrationthe filtrate is concentrated under vacuum
6. customto yield an oil
7. washwashed
8. dry with materialThe organic layer is dried over anhydrous Na2SO4
9. customthe solvent removed by evaporation
10. customThe crude product is purified by silica gel column