Sorbitol → L-Ascorbic Acid

HRID 1392001Notes

Reaction equation

HRID 1392001 reaction equation
[OH:1][CH2:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9]([CH2:11][OH:12])[OH:10])[OH:8])[OH:6])[OH:4].C([O-])([O-])=O.[Ca+2]>OCC(CO)O>[OH:8][C:7]1[C@@H:5]([C@@H:3]([OH:4])[CH2:2][OH:1])[O:6][C:11](=[O:12])[C:9]=1[OH:10]

Participants

4

Reactant 2

HCID 5780SorbitolHCID 10112Calcium Carbonate

Product 1

HCID 54670067L-Ascorbic Acid

Reagents, catalysts & solvents 1

HCID 753GlycerolSolvent

Procedure

3 ml of the seed culture were transferred into 500 ml Erlenmeyer flasks containing 50 ml of the production medium containing 8.0% D-sorbitol, 5.0% baker's yeast, 0.05% glycerol, 0.25% MgSO4.7H2O, 3.0% corn steep liquor, 1.5% CaCO3 and 0.15% antifoam. The cultivation was carried out at 30° C. with 180 rpm for 45 h on a rotary shaker. Then, the concentration of vitamin C produced was measured by HPLC at a wavelength of 264 nm with the system which was composed of a UV detector (TOSOH UV8000; TOSOH Co., Kyobashi 3-2-4, Chuo-ku, Tokyo, Japan), a dualpump (TOSOH CCPE; TOSOH Co.), an integrator (Shimadzu C-R6A; Shimadzu Co., Kuwahara-cho 1, Nishinokyo, Chukyo-ku, Kyoto, Japan) and a column (YMC-Pack polyamine II; YMC, Inc., 3233 Burnt Mill Drive Wilimington, N.C. 28403, USA), As a result, 118.1 mg/L of vitamin C was produced.

Workup

  1. additioncontaining 50 ml of the production medium

Sources & evidence

ORD record
ord-3949b92038974884af78e0dc6d9a755a
Source dataset
uspto-grants-2008_03
Patent number
US07341854B2

Reaction notes

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