反应详情

30 Milliliters of 20% hydrous pyridine was added to 1.12 g of 4-[(4,5-dihydro-1H-imidazol-2-yl)amino]benzoic acid.hydrochloride, 1.5 g of 6-amidino-1-(2-ethoxycarbonylethyl)-2-naphthol.hydrochloride, 1.44 g of DCC and 56.7 mg of DMAP, followed by stirring for 2 hours under cooling with ice and then 48 hours at room temperature. Furthermore, 1.44 g of DCC, 5 ml of 20% hydrous pyridine and 10 ml of DMF were added, followed by stirring for 24 hours at room temperature. Then, the precipitate was filtered and the filtrate was concentrated rated under reduced pressure. To the residue was added 20 ml of DMF, and the resulting solution was added dropwise to 400 ml of ether, followed by stirring for 24 hours under cooling with ice. The supernatant was decanted to obtain a crude product. This was subjected to silica gel column chromatography using methyl ethyl ketone-water-acetic acid (80:15:5) as an eluent, and the desired fractions were collected and the solvent was distilled off under reduced pressure. To the residue were added 10 ml of ethanol and 1.55 ml of concentrated hydrochloric acid, followed by stirring for 1 hour under cooling with ice. Then, the precipitate was collected by filtration to obtain 307.9 mg of the desired product.

1. temperatureunder cooling with ice
2. custom48 hours
3. customat room temperature
4. stirringby stirring for 24 hours at room temperature
5. filtrationThen, the precipitate was filtered
6. concentrationthe filtrate was concentrated
7. additionTo the residue was added 20 ml of DMF
8. additionthe resulting solution was added dropwise to 400 ml of ether
9. stirringby stirring for 24 hours
10. temperatureunder cooling with ice
11. customThe supernatant was decanted
12. customto obtain a crude product
13. customthe desired fractions were collected
14. distillationthe solvent was distilled off under reduced pressure
15. additionTo the residue were added 10 ml of ethanol and 1.55 ml of concentrated hydrochloric acid
16. stirringby stirring for 1 hour
17. temperatureunder cooling with ice
18. filtrationThen, the precipitate was collected by filtration