反应详情

温度

30 °C

Each strain was cultured with shaking in NB medium at 30° C. for 16 hours. For the culture of the transformant, spectinomycin was added to NB medium at a concentration of 100 μg/ml. Then, 0.5 ml of the seed culture was inoculated into 5 ml of a production medium (pH 7.0) comprising 80 g/l lactose, 40 g/l (NH4)2SO4, 0.5 g/l KH2PO4, 0.5 g/l K2HPO4, 100 mg/l L-arginine and 20 g/l CaCO3 in a test tube, and shaking culture was carried out at 30° C. for 72 hours. After the culturing was finished, the culture filtrate was subjected to paper chromatography and the amount of L-ornithine formed was determined colorimetrically by using the color development with ninhydrin. The results are shown in Table 20.

1. stirringshaking culture
2. waitwas carried out at 30° C. for 72 hours