反应详情

To a solution of 4-(5-phenyl-pentanoylamino)-thiophene-3-carboxylic acid methyl ester (50 mg, 0.16 mmol) in tetrahydrofuran/methanol 2/1 (1.5 ml) was added a 1 N aqueous LiOH solution (0.95 ml, 0.95 mmol). The reaction mixture was stirred at room temperature for 2 h, acidified with 1 N HCl and extracted two times with ethyl acetate. The combined organic layers were washed with water and brine and dried over sodium sulfate. The solvent was removed under reduced pressure and the crude product was purified by thin layer chromatography (SiO2, dichloromethane) to give the title compound as colorless oil (20 mg, 0.07 mmol, 42%).

1. extractionextracted two times with ethyl acetate
2. washThe combined organic layers were washed with water and brine
3. dry with materialdried over sodium sulfate
4. customThe solvent was removed under reduced pressure
5. customthe crude product was purified by thin layer chromatography (SiO2, dichloromethane)