Sodium Chloride → Sodium Lauryl Sulfate
Reaction equation
C1N(CCO)CCN(CC[S:12]([OH:15])(=[O:14])=[O:13])C1.[Na+:16].[Cl-].[Mg+2].[Cl-].[Cl-].C(S)[C@@H](O)[C@H](O)CS.C[C@H]1[C:35](=[O:36])[C:34](OC)=[C:33](OC)[C@H:32](O)[C@@H:31]1[CH2:42]/[CH:43]=[C:44](/[CH2:46][CH2:47]/[CH:48]=[C:49](/CCC=C(C)C)\C)\C>>[CH3:49][CH2:48][CH2:47][CH2:46][CH2:44][CH2:43][CH2:42][CH2:31][CH2:32][CH2:33][CH2:34][CH2:35][O:36][S:12]([O-:15])(=[O:13])=[O:14].[Na+:16]Participants
Procedure
In vitro prenylation reactions were performed in 20 μl reaction buffer (50 mM HEPES, pH 7.2, 50 mM NaCl, 5 mM MgCl2, 5 mM DTT, and 20 μM GDP) mixed with 3 μg FTase (Jena, Germany), 25 μM NBD-FPP, and 2 μg H-RasGST in the presence or absence of various concentrations of Antroquinonol. Reactions were incubated for 3 h at 37° C. and quenched by adding 20 μl 2×SDS-PAGE sample buffer and boiling at 95° C. for 3 min. Finally, the mixtures were resolved by 15% SDS-PAGE. The gel was scanned using a Typhoon 9400 scanner (GE Healthcare, UK) (excitation laser, 473 nm; emission cutoff filter, 510 nm) followed by staining with Coomassie blue. The fluorescent bands were quantified using Image-Pro Plus software (Media Cybernetics, Silver Spring, Md., USA).
Workup
- customquenched
- additionby adding 20 μl 2×SDS-PAGE sample buffer
- waitboiling at 95° C. for 3 min
- filtrationa Typhoon 9400 scanner (GE Healthcare, UK) (excitation laser, 473 nm; emission cutoff filter, 510 nm)
Sources & evidence
- ORD record
- ord-39fffd7795104987bf1739bf159db41f
- Source dataset
- uspto-grants-2016_08
- Patent number
- US09403747B2
Reaction notes
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