反应详情

温度

150 °C

A mixture of 3-amino-2-cyclopropyl-propionic acid tert-butyl ester (20 mg), 1-cyano-7-(2,6-difluoro-phenoxy)-4-hydroxy-isoquinoline-3-carboxylic acid ethyl ester (16 mg) and DBU (10 mg) in DMA (0.3 mL) was heated in an oil bath (150° C.) for 1 h. The reaction mixture was then partitioned between EtOAc and diluted HCl solution, EtOAc phase was separated and washed with water, diluted NaCl solution and dried over anhydrous sodium sulfate solution, filtered, concentrated and silica gel column purified to give desired product as brown solid (10 mg). LC-MS ESI+: 510 (M+1)+.

1. customThe reaction mixture was then partitioned between EtOAc and diluted HCl solution, EtOAc phase
2. customwas separated
3. washwashed with water, diluted NaCl solution
4. dry with materialdried over anhydrous sodium sulfate solution
5. filtrationfiltered
6. concentrationconcentrated
7. customsilica gel column purified