反应详情

Boc-Val-OH (8.69 g, 40.0 mmol) and Gly-OBzl.HCl (8.07 g, 40.0 mmol) were dissolved in methylene chloride (100 ml), and the solution was kept at 0° C. Triethylamine (6.13 ml, 44.0 mmol), HOBt (1-hydroxybenzotriazole, 6.74 g, 44.0 mmol) and WSC.HCl (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, 8.44 g, 44.0 mmol) were added to the solution, and the mixture was stirred overnight at room temperature. The reaction solution was concentrated under reduced pressure, and the residue was dissolved in ethyl acetate (200 ml). The solution was washed with water (50 ml), 5% of citric acid aqueous solution (50 ml× twice), saturated brine (50 ml), 5% of sodium hydrogen carbonate aqueous solution (50 ml× twice), and saturated brine (50 ml) again. The organic layer was dried over anhydrous magnesium sulfate, magnesium sulfate was removed by filtration, and the filtrate was concentrated under reduced pressure. The residue was recrystallized from ethyl acetate/n-hexane to obtain Boc-Val-Gly-OBzl (13.2 g, 36.2 mmol) as a white crystal.

1. customwas kept at 0° C
2. concentrationThe reaction solution was concentrated under reduced pressure
3. dissolutionthe residue was dissolved in ethyl acetate (200 ml)
4. washThe solution was washed with water (50 ml), 5% of citric acid aqueous solution (50 ml× twice), saturated brine (50 ml), 5% of sodium hydrogen carbonate aqueous solution (50 ml× twice), and saturated brine (50 ml) again
5. dry with materialThe organic layer was dried over anhydrous magnesium sulfate, magnesium sulfate
6. customwas removed by filtration
7. concentrationthe filtrate was concentrated under reduced pressure
8. customThe residue was recrystallized from ethyl acetate/n-hexane