Sucrose → HCID 19430206, compound

HRID 1500178Notes

Reaction equation

HRID 1500178 reaction equation
CC1C2C[C@@H:13]3[C@:19](O)(C(=O)C=2C(O)=C2C=1C=CC=C2O)[C:17](=O)[C:16](C(N)=O)=[C:15](O)[C@H:14]3[N:27]([CH3:29])[CH3:28].[CH2:32](O)[C@H:33]1[O:38][C@H:37]([O:39][C@:40]2([CH2:49]O)O[C@H](CO)[C@@H](O)[C@@H]2O)[C@H:36](O)[C@@H:35](O)[C@@H:34]1O.[CH2:55](S)[C@@H](O)[C@H](O)CS.C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=O>>[CH3:28][N:27]([CH3:29])[C:14]1[CH:15]=[CH:16][C:17]([C:32]2[CH:33]=[CH:34][CH:35]=[C:36]([C:37]([O:39][CH2:40][CH3:49])=[O:38])[CH:55]=2)=[CH:19][CH:13]=1

Participants

5

Reactant 4

HCID 5988SucroseHCID 6049Ethylenediaminetetraacetic AcidHCID 446094D-1,4-DithiothreitolHCID 178421650HCID 178421650, compound

Product 1

HCID 19430206HCID 19430206, compound

Procedure

For over-expression of ATGL and CGI-58 in E. coli (XL-1) cDNA was cloned into the vector pASK-IBA5+ (IBA-BioTagnology). Cells were transformed and cultured over night at 30° C. Thereafter cells were transferred into a fresh medium and grown until OD600 reached 0.7-0.8. Expression was induced using 0.2 μg/ml anhydrotetracycline. After 3 hours incubation at 37° C. cells where harvested, resuspended in lysis buffer (0.25M Sucrose, 1 mM DTT, 1 mM EDTA) and disrupted by sonication. Lysates were centrifuged at 15,000 g for 20 min at 4° C. and the supernatant was collected.

Workup

  1. waitcultured over night at 30° C
  2. customdisrupted by sonication
  3. waitLysates were centrifuged at 15,000 g for 20 min at 4° C.
  4. customthe supernatant was collected

Sources & evidence

ORD record
ord-58b3bef67f224f1cb97f23e9636c3a42
Source dataset
uspto-grants-2015_12
Patent number
US09206115B2

Reaction notes

Loading…