HRID1501922
反应详情
EQUATION
反应方程式
REACTANTS
反应物
PRODUCTS
生成物
AUXILIARIES
试剂、催化剂与溶剂
PROCEDURE
实验过程
Handling is carried out in a glove box ([O2]<50 ppm). A solution of TCEP at 300 mmol/L is prepared in 0.2 M phosphate buffer at pH=7.2. 3-Mercaptophenylacetic acid (MPAA) (84 mg, 0.5 mmol) is then added. The pH of the TCEP/MPAA solution obtained is adjusted to pH=6.5.
WORKUP
后处理
- customHandling is carried out in a glove box